Advanced Imaging Equipment
Leica SP8 & SP8X Confocal Microscopes
VBR houses two Leica TCS SP8 point-scanning confocal microscopes. Equipped with high efficiency Leica HyD detectors, prism/mirror-based spectral detection for custom emission band detection, Z-galvo stages for high speed scans in the Z-axis, and imaging options of standard confocal, FRET, and FRAP imaging. They are also equipped for brightfield, polarized, and DIC transmitted light imaging. In addition, one of the pair is equipped with the Leica white light laser system, for continous excitation wavelength choice from 470-670nm, and light gating capabilities.
Both microscope setups include Pathology Devices, LiveCell+ stage-top CO2, humidity, and temperature controlling incubators.
Leica SD6000 AF Spinning Disk Confocal
The Leica SD AF is an integrated system solution, combining a top-end Leica inverted research microscope, a Yokogawa spinning disk, and versatile Leica AF/MM MetaMorph software. The system includes a high-speed Hamamatsu Orca-R2 and two high-sensitivity Andor iXon3 cameras for fast confocal sectioning, 3D reconstruction, and TIRF microscopy techniques. The system is equipped with Warner Instrument's VC-6 six-channel perfusion system and TC-324B stage heater controller.
Manufacturer's Information: Leica SD6000 AF
The VivaView FL provides a fully integrated and motorized inverted microscope to allow high quality, long-term time-lapse imaging in a constant and optimized environment. Multiple locations in up to eight samples can be imaged simultaneously with fluorescence or Differential Interference Contrast. Simple, intuitive computer operation for easy configuration of the live cell imaging system. Other features include: powerful and proven image analysis with MetaMorph for Olympus; ability to load chemical solutions during growth; 40x/0.95na APO objective; 0.5/1.0/2.0 intermediate lens choice; 1W LED transmitted light/X-Cite with liquid light guide fluorescence illumination; and jog-dial focus control.
Manufacturer's Information: Olympus VivaView
IVIS Spectrum CT
IVIS (In Vivo Imaging System) is a novel technology that enables one to measure optical probes -- bioluminescence, fluorescence and Cerenkov imaging for radiotracers -- in live animals. Because the measurements are made on anesthetized animals, high precision longitudinal studies can be done with minimal numbers of test subjects and reagents, thus both reducing costs and increasing the validity of the results. The system is ideal for studies that follow the progress of tumor growth, spread, and regression; distribution and growth of infectious agents; and pharmacokinetic and pharmacodynamic studies with labelled pharmaceuticals.
The particular configuration of the IVIS instrument on the WSU campus is capable of measuring bioluminescent, fluorescent, and Cerenkov emissions from radio tracers in rodents and, in addition, has a CT (computed tomography) scanner built-in, and thus the location and intensity of the optical probes can be registered onto the animal’s skeleton in 3D.
Manufacturer's Information: IVIS Spectrum CT
The IVIS advanced imaging system was provided to the WSU research community by a generous grant from M. J. Murdock Charitable Trust. This donation will provide our researchers with a valuable tool, providing us with previously unavailable research techniques. Many thanks to the M. J. Murdock Charitable Trust.